PMC

a) Solid printed ABS, b) Designed sparse fill with IE-3076 with wollastonite additive, c) Hollow channels filled with IE-3076 with wollastonite additive, d) Hollow print filled with IE-3076 with wollastonite additive. The top plot shows the torque and rotational displacement of each sample during destructive testing.

Heterogenic expression of SPL2-GFP in bmh1∆ cells. a left panel, flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells (red) and bmh1Δ SPL2-GFP cells (blue) grown in YNB medium with 50 mM KCl; right panel, flow cytometry of BY4741 cells (line, no fill), bmh2Δ SPL2-GFP cells (red) and bmh1∆ SPL2-GFP cells (blue) grown in YNB medium with 50 mM KCl. b confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown in YNB medium with 50 mM KCl. Scale bar 10 μm. c confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown for 2 h in YNB medium without KCl. d confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown for 2 h in YNB medium without phosphate. e left panel: flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells grown in YNB medium with 50 mM KCl (blue) or without KCl (red). Right panel: flow cytometry of BY4741 cells (line, no fill), bmh1Δ SPL2-GFP cells grown in YNB medium with 50 mM KCl (blue) or without KCl (red). f left panel: flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells containing YCplac33 (blue) and BY4741 SPL2-GFP cells containing YCplac33[BMH1] (red) grown in YNB medium with 50 mM KCl. Right panel flow cytometry of BY4741 cells (line, no fill), bmh1Δ SPL2-GFP cells containing YCplac33 (blue) and bmh1Δ SPL2-GFP cells containing YCplac33[BMH1](red) grown in YNB medium with 50 mM KCl

Two example results of fill factor estimation function, . Left: Teli dataset; right: SONY dataset. The red stars show the estimated fill factors.

Effects of fill rate on bladder sensation–capacity curves A, with and B, without ultrasound. Sensation–capacity curves are shown for fill 1 (solid lines, slower filling) and fill 2 (dashed lines, faster filling). Increased fill rate led to decreased sensation (right shift) from 5% to 95% of capacity (P < 0.002; green-shaded regions; area under curve analysis; n = 10)